Dr. Wood’s Bioremediation & Applied Biotechnology Laboratory

Facilities

The 2500 ft^2Bioremediation & Applied Biotechnology Laboratory of Prof. Wood at the College Station Campus is a cutting-edge laboratory for genetic engineering techniques and characterization. This equipment and these facilities will be used for all of the experiments in the PI’s lab. (Rooms 502/504/506/508 at Jack.E.Brown Engineering Building).

Computers

Intranet of 15 personal computers with word processing, SigmaPlot graphics, VECTOR NTI, and spreadsheet analysis, a HP LaserJet printer, a LaserWriter Select 360 printer, 2 Epson color printers, and two Hewlet Packard inkjet printers.

Major Equipments

For biofilms

For culturing bacteria and handling biomaterials

For molecular biology

For chemical analysis

For DNA huffling

For monitoring corrosion with biofilms

· Equipment for biofilms: Back to top

v Complete Affymetrix microarray system consisting of Ø A GeneChip Fluidics Station 450 (Fig 1.) Ø A GeneChip Hybridization oven 640 (Fig 2.) Ø A laser scanner (Fig 3.)

Fig 1

Fig 2

Fig 3

v

v Two Leica TCS SP5 confocal microscopes for viewing flow cell biofilms (one with a resonant scanner for fast imaging) and IMARIS confocal software

v Two Robbins devices, Two flow cells (model FC81, Biosurface Technologies Corp)

· Equipment culturing bacteria and handling biomaterials: Back to top

v A 2-liter New Brunswick BioFlow III Fermentor linked to a personal computer (Fig.4) v One Applikon 2-liter fermentors with controller (in Fig.5) v Three 1.5 liter fixed-film glass biofilters (in Fig.5) v Two 1.0 liter fluidized-bed reactors, six IKA shakers v An Amicon ultrafiltration unit

Fig. 4

Fig.5

v Two Beckman J2-HS floor centrifuges

v 6 chemical fume hoods

v Three New Brunswick G25 floor shakers

v Six incubators, four refrigerators, three -80°C freezers, and two -20°C freezers

v A French Press

v Two 8.8 ft^3 upright AMSCO autoclaves

· Equipment for molecular biology: Back to top

v Bio-Rad Gene Pulser and Pulse Controller for electroporation of plasmid DNA

v Electrophoretic transfer cell

v Polaroid MP-4 camera with transilluminator

v Savant SpeedVac

v Microcentrifuges

v Other equipment (pictures temporarily unavailable): Ø Access to Vector NTI DNA software Ø 13 Pipetman Ø 3 horizontal and 1 vertical electrophoresis units Ø A dot-blot apparatus Ø A rotating hybridization chamber Ø Two power supplies Ø An electroelution device Ø A Peltier hot block

· Equipment for chemical analysis: Back to top

v Shimadzu UV1601 spectrophotometer

v 5890A Series II GC (ECD/FID) with PC-controlled ChemStation data acquisition

v Hewlet Packard 6890 GC (ECD, FID) with PC-controlled ChemStation data acquisition

v Waters Model 515 HPLC with photodiode array detector, 717+ autosampler, and PC-controlled data acquisition

v Cary 1E UV-Vis spectrophotomer

v Shimadzu Mini UV1240 spectrophotometer

v Other equipment (pictures temporarily unavailable): Ø A Gilson 118/805/811C/306 UV-Vis HPLC Ø An ion chromatograph Ø A YellowSprings Instrument Biological Oxygen Monitor Ø A Labconco rotary evaporator Ø Two refrigerated/heated Fisher Isotemp water circulators Ø A Beckman DU 640 UV/Visible Spectrophotometer

· Equipment for DNA shuffling: Back to top

v One Perkin-Elmer Thermal Cycler for polymerase-chain reaction techniques, two MJ Research MiniCyclers for polymerase-chain reaction techniques, two Millipore vacuum manifolds for filtering 96-well plates

v Labsystems Multiskan RC plate reader

· Equipment for monitoring corrosion with biofilms: Back to top

v A BioAnalytical Systems IM6 Impedance Analyzer with 16-channel cell multiplexer and noise probe interfaced to a Gateway computer, and eight autoclavable continuous reactors which may operated simultaneously

Others

Additional biotechnological and environmental equipment is available at in the Chemical Engineering Department which houses the labs of 3 other biotechnologists.